Year : 2009  |  Volume : 13  |  Issue : 2  |  Page : 84--87

Rapid method for the determination of some organophosphorus insecticides in a small amount of serum in emergency and occupational toxicology cases


Bhoopendra Singh, TD Dogra 
 Department of Forensic Medicine & Toxicology, All India Institute of Medical Sciences, New Delhi - 110029, India

Correspondence Address:
Bhoopendra Singh
Department of Forensic Medicine & Toxicology, Rural Medical College, Pravara Institute of Medical Sciences (Deemed University), Loni - 413736, Ahmednagar, Maharashtra
India

A simple and rapid method is described for the estimation of some organophosphorus insecticides in the serum of occupationally exposed persons. The compounds are extracted with a mixture of acetone and diethyl ether (1:1 v/v) in acidic medium and the extraction residue is analyzed by gas chromatography with nitrogen phosphorus detection method. Linearity was acceptable over concentrations from 0.25 to 4.0 g/mL. The method percentile recovery for the six different organophosphorus insecticides was 86.3% for phorate, 78.3% for dimethoate, 82.3% for malathion, 79.4% for chlorpyrifos, 80.2% for diazinon, and 68.5% for ethion at the g/mL level. Serum samples of nine workers who had been occupationally exposed to malathion in an insecticide manufacturing factory, were analyzed and malathion was found at low levels in all the samples.


How to cite this article:
Singh B, Dogra T D. Rapid method for the determination of some organophosphorus insecticides in a small amount of serum in emergency and occupational toxicology cases.Indian J Occup Environ Med 2009;13:84-87


How to cite this URL:
Singh B, Dogra T D. Rapid method for the determination of some organophosphorus insecticides in a small amount of serum in emergency and occupational toxicology cases. Indian J Occup Environ Med [serial online] 2009 [cited 2022 Oct 5 ];13:84-87
Available from: https://www.ijoem.com/article.asp?issn=0973-2284;year=2009;volume=13;issue=2;spage=84;epage=87;aulast=Singh;type=0